One of a kind twin indexes cut down for every-sample Price tag and sequence libraries with the best accuracy as compared to other indexing procedures.
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The MiniSeq Speedy designed a recipe alter that employs the grafted oligo for that i5 index priming, so it is go through inside the ahead orientation comparable to a MiSeq.
csv file or perhaps the SampleSheet.csv file produced by LRM for the Examination start. LRM will detect the instrument variety in the run information and make a final sample sheet Along with the i5 indexes in the proper orientation for that platform.
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When evaluating index sets for color equilibrium, the sequence observed with the instrument have to be assessed, regardless of the orientation employed for the sample sheet. With the i7 index, normally asses colour stability utilizing the "i7 bases for sample sheet" column orientation.
For runs uploaded manually (i.e., an Illumina tool is not getting used to crank out the sample sheet), the index orientation will depend on which instrument you will be working with. Remember to see the Index Orientation Desk for info on the best way to enter the i5 sequence depending upon the instrument and program utilised.